The SARS-CoV-2 Spike Protein Serological IgA ELISA Kit is a solid-phase ELISA that detects binding of human IgA to full-length ectodomain SARS-CoV-2 spike trimeric protein (S-protein). Trimeric spike protein has been coated onto microwells. After incubation with sample, human IgA specific for trimeric spike protein is captured on the plate. The wells are then washed to remove unbound material. Anti-Human IgA, HRP-linked antibody is then used to recognize the bound IgA. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of IgA specific for spike protein.
*Antibodies in this kit are custom formulations specific to kit. -
