The PathScan® Total Vimentin Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total vimentin protein. A Vimentin Mouse mAb has been coated onto the microwells. After incubation with cell lysates, both phospho- and nonphospho-vimentin protein is captured by the coated antibody. Following extensive washing, a Vimentin Rabbit Detection Antibody is added to detect the captured vimentin protein. Anti-rabbit IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of vimentin protein.
*Antibodies in this kit are custom formulations specific to kit. -
