The PathScan® Total Histone H3 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of histone H3. A histone H3 rabbit antibody has been coated onto the microwells. After incubation with cell lysates, histone H3 (modified and unmodified) is captured by the coated antibody. Following extensive washing, histone H3 mouse mAb is added to detect the captured histone H3 protein. Anti-Mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total histone H3.
*Antibodies in this kit are custom formulations specific to kit. -
