PathScan® Total Ezh2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total Ezh2 protein. An Ezh2 specific antibody has been coated onto the microwells. After incubation with cell lysates, Ezh2 protein is captured by the coated antibody. Following extensive washing, an Ezh2 mouse mAb is added to detect the captured Ezh2 protein. Anti-Mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of total Ezh2 protein.
*Antibodies in this kit are custom formulations specific to kit. -
