The PathScan® Total EpCAM Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of EpCAM protein. An EpCAM rabbit mAb has been coated onto the microwells. After incubation with cell lysates, the EpCAM proteins are captured by the coated antibody. Following extensive washing, an EpCAM mouse detection mAb is added to detect captured EpCAM proteins. Anti-mouse, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of EpCAM protein.
*Antibodies in this kit are custom formulations specific to kit. -
