The rapid protocol PathScan® RP Phospho-VEGFR-2 (Tyr1175) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Phospho-VEGFR-2 (Tyr1175) protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with VEGFR2 protein phosphorylated at Tyr1175 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of VEGFR2 protein phosphorylated at Tyr1175. Learn more about all of your ELISA kit options here.
*Antibodies in kit are custom formulations specific to kit. -
