The PathScan® Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that recognizes endogenous levels of phospho-SMAD2 (Ser465/467) and SMAD3 (Ser423/425) proteins. A SMAD2/3 Mouse Antibody has been coated on the microwells. After incubation with cell lysates, SMAD2/3 proteins (phospho and nonphospho) are captured by the coated antibody. Following extensive washing, a Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) Detection Antibody is added to detect captured phospho-SMAD2 (Ser465/467) and phospho-SMAD3 (Ser423/425) proteins. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-SMAD2 (Ser465/467) and phospho-SMAD3 (Ser423/425) proteins.
*Antibodies in this kit are custom formulations specific to kit. -
