The PathScan® Phospho-ROS1 (panTyr) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tyrosine-phosphorylated ROS1 protein. A ROS1 rabbit antibody has been coated onto the microwells. After incubation with cell lysates, ROS1 protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a phospho-tyrosine mouse detection antibody is added to detect captured tyrosine-phosphorylated ROS1 protein. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of ROS1 protein phosphorylated on tyrosine.
*Antibodies in this kit are custom formulations specific to kit. -
