The PathScan® Phospho-MLKL (Ser358) Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of MLKL protein phosphorylated at Ser358. A phospho-MLKL (Ser358) rabbit antibody has been coated onto the microwells. After incubation with cell lysates, MLKL protein phosphorylated at Ser358 is captured by the coated antibody. Following extensive washing, an MLKL mouse detection antibody is added to detect the captured phospho-MLKL (Ser358) protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of phospho-MLKL (Ser358) protein. *Antibodies in this kit are custom formulations specific to kit. -
