The PathScan® Phospho-Histone H2A.X (Ser139) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of histone H2A.X protein phosphorylated at Ser139. A histone H2A.X rabbit antibody has been coated onto the microwells. After incubation with cell lysates, both phospho- and non-phospho-histone H2A.X proteins are captured by the coated antibody. Following extensive washing, a phospho-histone H2A.X (Ser139) mouse antibody is added to detect the captured phospho-histone H2A.X protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of histone H2A.X phosphorylated at Ser139.
*Antibodies in this kit are custom formulations specific to kit. -
