The PathScan® Phospho-HER3/ErbB3 (Tyr1328) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of HER3/ErbB3 protein phosphorylated at Tyr1328. A HER3/ErbB3 mouse antibody has been coated onto the microwells. After incubation with cell lysates, HER3/ErbB3 protein is captured by the coated antibody. Following extensive washing, a phospho-HER3/ErbB3 (Tyr1328) rabbit detection antibody is added to detect the captured phospho-HER3/ErbB3 (Tyr1328) protein. Anti-rabbit IgG HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for this developed color is proportional to the quantity of HER3/ErbB3 protein phosphorylated at Tyr1328.
*Antibodies in this kit are custom formulations specific to kit. -
