The PathScan® Phospho-EGF Receptor (panTyr) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tyrosine-phosphorylated EGF receptor protein. An EGF Receptor Rabbit mAb has been coated on the microwells. After incubation with cell lysates, EGF receptor protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Phospho-Tyrosine Mouse Detection mAb is added to detect captured tyrosine-phosphorylated EGF receptor protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of EGF receptor protein phosphorylated at tyrosine residues.
*Antibodies in this kit are custom formulations specific to kit. -
