The PathScan® Phospho-CrkL (Tyr207) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of CrkL protein phosphorylated at Tyr207. A phospho-CrkL (Tyr207) rabbit antibody has been coated onto the microwells. After incubation with cell lysates, phospho-CrkL (Tyr207) protein is captured by the coated antibody. Following extensive washing, a biotinylated CrkL rabbit detection antibody is added to detect the captured phospho-CrkL (Tyr207) protein. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of CrkL protein phosphorylated at Tyr207.
*Antibodies in this kit are custom formulations specific to kit. -
